槐耳浸膏联合化疗药物对人胃癌MGC803的体外抑制作用

周玲,乐涵波

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (18) : 1607-1612.

PDF(1051 KB)
PDF(1051 KB)
中国药学杂志 ›› 2015, Vol. 50 ›› Issue (18) : 1607-1612. DOI: 10.11669/cpj.2015.18.007
论 著

槐耳浸膏联合化疗药物对人胃癌MGC803的体外抑制作用

  • 周玲a,乐涵波b
作者信息 +

Inhibition Effect of Extractum Robinia-living trametes Combined with Chemotherapy on Proliferation of Gastric Cancer Cell MGC803 in Vitro

  • ZHOU Linga,YUE Han-bob*
Author information +
文章历史 +

摘要

目的 筛选出槐耳浸膏与化疗药物的最佳组合并探讨槐耳浸膏和化疗药物5-氟尿嘧啶诱导人胃癌MGC803细胞凋亡的机制。方法 以不同浓度槐耳浸膏和化疗药物(5-氟尿嘧啶,紫杉醇)作用于体外培养的人胃癌细胞MGC803,通过 MTT 法检测槐耳浸膏对MGC803细胞增殖的作用,倒置显微镜观察其形态的变化;将0.2 mgmL-1的槐耳浸膏分别与5-FU(2.5 μgmL-1)或紫杉醇(0.5 μgmL-1)联合处理细胞,比较存活率。流式细胞术检测0.2 mgmL-1槐耳浸膏联合5-FU(2.5 μgmL-1)干预MGC803细胞24、48 h后细胞的凋亡情况;Western Blot法检测0.2 mgmL-1槐耳浸膏联合5-FU(2.5 μgmL-1)干预MGC803细胞24、48 h后细胞的P53、T-Akt和P-Akt蛋白的表达。结果 槐耳浸膏及化疗药物(5-氟尿嘧啶,紫杉醇)均能降低MGC803细胞存活率,并呈时间和剂量依赖性;倒置显微镜下可见细胞胞体缩小、核碎裂,且呈浓度与时间依赖性(P<0.01)。相较于单独应用化疗药物,联合用药能够显著降低细胞的生存率。槐耳浸膏联合5-FU联合给药上调人胃癌细胞MGC803 T-Akt及P53蛋白表达量,同时下调P-Akt的表达量。结论 槐耳浸膏联合5-FU在体外诱导胃癌细胞MGC803凋亡作用可能是通过抑制PI3K/Akt信号通路来实现的。联合使用槐耳浸膏和化疗药物可能会更有效发挥槐耳抗肿瘤增殖的作用。

Abstract

OBJECTIVE To screen the best combination of extractum of Robinia-living trametes and chemotherapy and investigate the action mechanism of Robinia-living trametes against the apoptosis of human gastric cancer cell MGC803. METHODS MGC803 Cells were treated with different concentrations of Robinia-living trametes and chemotherapy drugs (5-Fu and paclitaxel) in vitro. The inhibitory rate of cells was measured by MTT assay. Morphological changes were observed with inverted microscope. The apoptosis rate of MGC803 cells which were treated with combination of Robinia-living trametes(0.2 mgmL-1) and 5-Fu (2.5 μgmL-1) was detected by FCM. The protein expression of P53 and p-Akt in MGC803 cells which were treated with combination of Robinia-living trametes (0.2 mgmL-1) and 5-Fu (2.5 μgmL-1) was detected by Western blot. RESULTS The viability of MGC803 cells was reduced by Robinia-living trametes and chemotherapy drugs (5-Fu and paclitaxel) in a concentration- and time-dependent manner(P<0.01). Under reverse microscopy, cell body shrinking, nuclear pyrosis, and nuclear fragmentation were observed. The higher concentration, the longer treatment time, the more cells died. Compared with monotherapy, the combination of Robinia-living trametes and chemotherapy could reduce the survival rate of MGC803 cells. The protein expressions of P53 in MGC803 cells treated with combination of drugs was up-regulated, while that of P-Akt was down-regulated. CONCLUSION The apoptosis of MGC803 cells in vitro may be induced by the inhibitory effect of the combination of Robinia-living trametes and 5-Fu on PI3K/Akt signaling pathway. Combination therapy of Robinia-living trametes and 5-Fu is potentially more effective in inhibition of tumor cells than monotherapy of Robinia-living trametes.

关键词

槐耳 / 紫杉醇 / 人胃癌细胞MGC803 / 细胞凋亡 / P53蛋白 / P-Akt蛋白 / T-Akt蛋白

Key words

Robinia-living trametes / paclitaxel / human gastric cancer cell MGC803 / apoptosis / P53 / P-Akt / T-Akt

引用本文

导出引用
周玲,乐涵波. 槐耳浸膏联合化疗药物对人胃癌MGC803的体外抑制作用[J]. 中国药学杂志, 2015, 50(18): 1607-1612 https://doi.org/10.11669/cpj.2015.18.007
ZHOU Ling,YUE Han-bo. Inhibition Effect of Extractum Robinia-living trametes Combined with Chemotherapy on Proliferation of Gastric Cancer Cell MGC803 in Vitro[J]. Chinese Pharmaceutical Journal, 2015, 50(18): 1607-1612 https://doi.org/10.11669/cpj.2015.18.007
中图分类号: R969   

参考文献

[1] XIE R J,ZHANG Y,CUI X L. Advances in gastric cancer prevention and control by traditional Chinese medicine [J]. Inf Tradit Chin Med(中医药信息),2008,25(5):15-16.
[2] FANG X P. The treatment of apoptosis-inducing on gastric cancer [J]. J China Traditi Chin Med Inf(中国中医药咨讯),2011,3(4):212.
[3] WANG X,ZHANG N,HUO Q,et al.Antiangiogenic and antitumor activities of Huaier aqueous extract[J]. Oncol Rep,2012,28(4):1167-1175.
[4] ZHANG N,KONG X,YAN S,et al. Huaier aqueous extract inhibits proliferation of breast cancer cells by inducing apoptosis[J]. Cancer Sci,2010,101(11):2375-2383.
[5] ZHUANG Y. Characterization and textual criticism of huai er[J]. Edib Fungi China(中国食用菌),1993,13(6):22.
[6] ZHENG Y H,LIU Y J,JING Q,et al. The analysis of the therapeutical effects of jew ear parasitized granula to primary hepatic carcinoma (PHC) [J]. Med Inf(医学信息),2006,19(10) :1815-1817.
[7] LI X B. Effects of Jinke Huaier granule on immune cell activity in patients with Stage Ⅲ non-small cell lung cancer[J]. J Clin Pulm Med(临床肺科杂志),2006,11(4):472-473.
[8] DENG A P,MAO D L. Seventy-four clinical researches of Huaier granules in the treatment of gastrointestinal cancers[J]. Chin J Hosp Pharm(中国医院药学杂志),2005,25(5):453-454.
[9] SONG G,OUYANG G L,BAO S D. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med,2005,9 (1):59-71.
PDF(1051 KB)

Accesses

Citation

Detail

段落导航
相关文章

/